Forensic Biology and DNA Analysis – Blood, Semen, and Skeletal Remains Practice Exam

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What indicates a positive acid phosphate test?

The solution turns orange.

A purple color appears when the test solution is added.

The test is measuring acid phosphatase enzyme activity, which is abundant in semen. When a substrate for acid phosphatase is present, the enzyme converts it into a colored product. A purple color after adding the test reagents indicates that acid phosphatase is present, signaling a positive result consistent with semen. If there’s no color change, or the solution only turns orange or forms a precipitate, those outcomes do not indicate a positive ACP result under the standard procedure.

No color change is observed.

The test forms a precipitate.

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